Cryopreserved Human Precision-Cut Lung Slices as a Bioassay for Live Tissue Banking. A Viability Study of Bronchodilation with Bitter-Taste Receptor Agonists
نویسندگان
چکیده
منابع مشابه
Biaxial distension of precision-cut lung slices.
The mechanical forces acting on lung parenchyma during (mechanical) ventilation and its (patho)physiological consequences are currently under intense scrutiny. Several in vivo and cell culture models have been developed to study the pulmonary responses to mechanical stretch. While providing extremely useful information, these models do also suffer from limitations in being either too complex fo...
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1.When the immune system encounters incoming infectious agents, this generally leads to immunity. The evoked immune response is usually robust, but can be severely perturbed by potentially harmful environmental agents such as chemicals, pharmaceuticals and allergens. 2.Immunosuppression, hypersensitivity and autoimmunity may occur due to changed immune activity. Evaluation of the immunotoxic po...
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Several studies have shown that genetic factors account for 25% of the variation in human life span. On the basis of published molecular, genetic and epidemiological data, we hypothesized that genetic polymorphisms of taste receptors, which modulate food preferences but are also expressed in a number of organs and regulate food absorption processing and metabolism, could modulate the aging proc...
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The precision-cut lung slice (PCLS) technique is widely used to examine airway responses in different species. We developed a method to study nerve-dependent bronchoconstriction by the application of electric field stimulation (EFS) to PCLS. PCLS prepared from Wistar rats were placed between two platinum electrodes to apply serial rectangular impulses (5-100 Hz), and bronchoconstriction was stu...
متن کاملCharacterization of guinea pig precision-cut lung slices: Comparison with human tissues
Precision-cut lung slices (PCLS) allow comparison of airway responses of different species under identical experimental conditions. Our aim was to establish and characterize PCLS from guinea pigs (GP) and to compare them to human PCLS. Guinea pig PCLS were prepared according to previously published procedures with the exception that the agarose solution and the initial incubation medium contain...
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ژورنال
عنوان ژورنال: American Journal of Respiratory Cell and Molecular Biology
سال: 2016
ISSN: 1044-1549,1535-4989
DOI: 10.1165/rcmb.2015-0290ma